Cell· Donghui Li, Alberto De Iaco, Kevin C. Ngan, Georgy Smyshlyaev, Ninad Kumbhojkar, Barrett E. Steinberg, Zohra Kalani, Lingbo Xia, Alexas Dolbee, Emily S. Kibbler, Miles C. Duncan, Ian Lin, Matthew S. Benczkowski, Lavanya Venkatesan, Andrea Calabria, Thomas A. Carter, Giulia I. Corsi, Trine A. Kristiansen, Gregory McAllister, Christopher Chase Bolt, Kimberly A. Harris, Jeffrey I. Boucher, Chunxi Zeng, Isha S. Dakhara, Xijia Wang, Shanmugapriya Sothiselvam, Ersin Akinci, Justin Strickland, Anubhuti Mathur, Christina Ferren, Jessica Desmond, Jason Rodriguez, James B. Rottman, Mohit Gupta, Lei Liu, Rahul Palchaudhuri, Z. Jane Wang, Robert J. Citorik, Jacob R. Rubens, Narahari S. Pujar, Michael C. Holmes, Cecilia Cotta-Ramusino·· 18 小时前精选评分80
工程化逆转座子Gene Writers在体内写入基因并生成功能性CAR-T细胞
In vivo Gene Writing with engineered retrotransposons
导读
研究通过计算重构、筛选和改造非LTR逆转座子，得到可在人类细胞中经全RNA递送稳定整合转基因的Gene Writers。经过表征和优化后，Gene Writers在人源化小鼠模型中于体内生成了功能性CAR-T细胞。
推荐理由
从计算重构与筛选优化，到全RNA递送后在人源化小鼠中生成功能性CAR-T，验证链条可供基因写入研究借鉴。
来源：Cell