Cell· Donghui Li, Alberto De Iaco, Kevin C. Ngan, Georgy Smyshlyaev, Ninad Kumbhojkar, Barrett E. Steinberg, Zohra Kalani, Lingbo Xia, Alexas Dolbee, Emily S. Kibbler, Miles C. Duncan, Ian Lin, Matthew S. Benczkowski, Lavanya Venkatesan, Andrea Calabria, Thomas A. Carter, Giulia I. Corsi, Trine A. Kristiansen, Gregory McAllister, Christopher Chase Bolt, Kimberly A. Harris, Jeffrey I. Boucher, Chunxi Zeng, Isha S. Dakhara, Xijia Wang, Shanmugapriya Sothiselvam, Ersin Akinci, Justin Strickland, Anubhuti Mathur, Christina Ferren, Jessica Desmond, Jason Rodriguez, James B. Rottman, Mohit Gupta, Lei Liu, Rahul Palchaudhuri, Z. Jane Wang, Robert J. Citorik, Jacob R. Rubens, Narahari S. Pujar, Michael C. Holmes, Cecilia Cotta-Ramusino·· 19 hours agoPickScore80
In vivo Gene Writing with engineered retrotransposons
In brief
Researchers computationally reconstructed, screened, and engineered non-LTR retrotransposons to obtain Gene Writers that achieve stable transgene integration in human cells via all-RNA delivery. After characterization and optimization, Gene Writers generated functional CAR-T cells in vivo in a humanized mouse model.
Why it matters
The workflow, from computational reconstruction and screening to in vivo CAR-T generation in humanized mice, offers a template for gene writing research.
Source: Cell