bioRxiv· Wen, J., Del Bel Belluz, L., Ottaviani, S., Poyia, F., Yu, H., Sachdev, S., El Eid, L., Garner, K., Mistry, K., Hayes, E., Moderau, N., Chandrasinghe, P. C., Stebbing, J., Cereser, B. ·· 1 day agoPickScore63
Cytoplasmic localization of 5hmC reveals RNA hydroxymethylation in breast cancer
In brief
Wen et al. report in a bioRxiv preprint a quantifiable cytoplasmic 5hmC signal in breast cancer cell lines and human breast cancer tissue. RNase-sensitive, RNA-specific ELISA confirmed the signal reflects total RNA and mRNA hydroxymethylation, enriched in triple-negative (ER-/PR-/HER2-) breast cancer and grade 3 tumors, and correlated with increased cytoplasmic TET2/TET3.
Why it matters
Tissue signal assessed by digital pathology and confirmed as total RNA and mRNA hydroxymethylation by RNase-sensitive, RNA-specific ELISA, providing a method reference for similar assays.
Source: bioRxiv