bioRxiv Genomics· Cintori, L., Chailleux, C., Maller, P., di Guilmi, A.-M., Siberchicot, C., Caetano, T., Lehir, E., Jouffret, V., Reyes, C., Aguirrebengoa, M., Campalans, A., Bergoglio, V., Trouche, D., Canitrot, Y. ·· 23 hours agoPickScore69
Direct effects on gene transcription revealed by global and CRISPR-targeted oxidative DNA damage
In brief
Oxidative stress rapidly represses or activates many genes and upregulates many read-through RNAs, and early gene repression partially depends on OGG1-mediated repair processing of oxidized DNA. Total RNA-Seq and ChIP-seq of RNA polymerase II show that recruitment of RNA polymerase II to transcription start sites changes under oxidative stress.
Why it matters
Targeting ROS to designated genomic loci combined with OGG1 degradation provides a design reference for dissecting how oxidative DNA damage affects promoter transcription.
Source: bioRxiv Genomics · biorxiv.org