Nat Biotechnol·· 8 hours agoPickScore81
Deep dynamical models of single-cell multiomic velocities predict loss-of-function and rescue perturbations in B cells.
In brief
DynaVelo predicts loss-of-function perturbations in B cells from single-cell multiome data and identifies Ctcf, Stat3 and Bcl6 as rescue targets for Arid1a loss. The generative neural ordinary differential equation model recovers the bifurcating in vivo dynamics of wild-type murine germinal center B cells and reveals changes in dynamics and gene regulatory networks under heterozygous loss of Arid1a and Ctcf.
Why it matters
DynaVelo couples single-cell multiomic dynamics with in silico perturbation to predict B cell regulatory networks and rescue targets for Arid1a loss, validated in double-heterozygous mice.
Source: Nat Biotechnol · doi.org