Nature· Christopher D. Reinkemeier·· 13 hours agoPickScore75
Recordanelles for transcriptome-wide single-cell RNA recording in human cells
In brief
This paper reports a synthetic organelle termed the recordanelle that continuously samples and protects endogenous mRNAs in HEK293T cells, enabling reconstruction of transcriptional events at single-cell level after IFNβ stimulation is withdrawn.
Why it matters
The paper builds the recordanelle tool and provides control data showing minimal perturbation of the transcriptome, proteome and mitochondrial function.
Source: Nature