two years after alphafold won the nobel, how much has it changed your actual lab work?

A poster in r/Biochemistry (u/Euphoric-Disaster119) asked, two years after the 2024 Nobel Prize in Chemistry (David Baker for computational protein design; Demis Hassabis and John Jumper for protein structure prediction): to what extent has AlphaFold actually changed everyday experimental work? The comments offer pragmatic assessments from frontline researchers: u/ProfPathCambridge (87▲) said that by the time of publication "designing mutations for a specific purpose" had already become routine, but most designed mutations don't work and still require comprehensive screening; u/sofia-online noted that structural phylogenetics is now feasible when it previously wasn't; u/Pink_Moone-stone, u/superhelical and others agreed that "predicted structures are now the standard first step in structural biology workflows, but flexible regions, complexes, and ligand-binding sites still require validation by cryo-EM/X-ray/NMR." On the factual level, it is confirmed that the 2024 Nobel Prize in Chemistry was awarded for AlphaFold and protein design. On the speculative level, the discussion of "how reliable predictions are on difficult cases" reflects researchers' experiential judgments.

Checked ✔ Verified: The 2024 Nobel Prize in Chemistry was awarded to