Aseptic Technique Prep: Bench Cleaning, Glove and Bottle Disinfection, and Hood UV (Addgene Quick Tips)
Lab coats and gloves protect the operator and keep skin flakes and debris out of samples; check wrists and forearms for exposed skin and add sleeve covers, masks, or hair nets if needed. Spray the bench, gloves, sleeves, and all bottle and container surfaces with isopropanol (or slightly diluted ethanol) and let them dry fully. Spray thoroughly inside the hood as well; its UV lamp is a secondary measure only. This is a roughly 3-minute introductory protocol video for molecular biology and plasmid work, not a research finding.
Start with the person: lab coats and gloves protect the operator, but also prevent the operator from introducing skin flakes/debris into the sample; after putting on gloves, check wrists/forearms for exposed skin (some people shed skin flakes more easily), and if necessary add disposable sleeve covers, a mask, or a hair net.; Surface disinfection: spray the benchtop, gloves, sleeves, and the exterior of all bottles/containers with isopropanol (or slightly diluted ethanol) and let it dry completely before working (avoid carrying alcohol into the sample; wet bottles become slippery, so grip the bottom for stability).; Biosafety cabinet: inside the hood, thorough spraying is likewise required first; the airflow is strong, so it dries faster; the in-hood UV lamp can serve as a secondary measure, but spraying must still come first.; Positioning: introductory operational guidelines for molecular biology/plasmid experiments (a roughly 3-minute short video), not research findings.
- Start with the person: lab coats and gloves protect the operator, but also prevent the operator from introducing skin flakes/debris into the sample; after putting on gloves, check wrists/forearms for exposed skin (some people shed skin flakes more easily), and if necessary add disposable sleeve covers, a mask, or a hair net.
- Surface disinfection: spray the benchtop, gloves, sleeves, and the exterior of all bottles/containers with isopropanol (or slightly diluted ethanol) and let it dry completely before working (avoid carrying alcohol into the sample; wet bottles become slippery, so grip the bottom for stability).
- Biosafety cabinet: inside the hood, thorough spraying is likewise required first; the airflow is strong, so it dries faster; the in-hood UV lamp can serve as a secondary measure, but spraying must still come first.
- Positioning: introductory operational guidelines for molecular biology/plasmid experiments (a roughly 3-minute short video), not research findings.
Source: YouTube