Cell· Drew W. Grant, Shengjiang Tan, Dylan E. Ramage, Mamie Z. Li, Ying Di, Iva A. Tchasovnikarova, Michael P. Weekes, Stephen J. Elledge, Alan J. Warren, Richard T. Timms·· 18 hours agoPickScore61
Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis
In brief
Grant et al. used ORFeome libraries with different C-terminal architectures for comparative stability profiling and identified a conditionally regulated C-degron pathway governed by the CTLH/Muskelin E3 ubiquitin ligase complex. Its substrate AAMP is the human ortholog of yeast Sqt1 and acts as a uL16 chaperone, linking nutrient status to the regulation of ribosome biogenesis.
Why it matters
Comparative stability profiling with ORFeome libraries of different C-terminal architectures identifies AAMP as a CTLH E3 complex substrate and links nutrient status to ribosome biogenesis.
Source: Cell