biorxiv.org· Huang, X., Berkemeier, F., Berners-Lee, R., Sherborne, K., Chapman, S. E., Merrick, C. J., Boemo, M. A. ·· 1 day agoPickScore48
Nanopore sequencing reveals the DNA replication timing programme of the malaria parasite Plasmodium falciparum
Nanopore sequencing reveals the DNA replication timing programme of the malaria parasite Plasmodium falciparum
In brief
Oxford Nanopore long-read sequencing with DNAscent provides the first replication timing programme for Plasmodium falciparum, which Repli-seq cannot address because of the parasite's unusual cell cycle. The work shows that replication timing relates to elevated rates of DNA breaking and replication fork stalling, and predicts that these stalls are predominantly transient.
Why it matters
First replication timing map for a major pathogen whose cell cycle blocks standard Repli-seq methods.
Source: biorxiv.org · biorxiv.org